Project C4

Predicting molecular mechanisms of adaptation to chemoradiation therapy in cancer

Axel Hillmer, U Cologne | web | email
Reinhard B├╝ttner, U Cologne | web | email

We use a xenograft mouse model for adaptation and evolution of esophageal adenocarcinoma (EAC) genomes to chemoradiation therapy (RCT) to predict the genome-rearrangement dynamics in response to RCT, to model the cost of BRCA2 loss, and to define essential genes for acquisition of resistance. To achieve this, we are using a combination of single cell RNA and bulk whole genome sequencing and perform CRISPR/Cas9 screens to identify essential genes for RCT resistance. We work closely with projects C2 and Z3.

Predictability in Evolution

Collaborative Research Center 1310